Human VEGF(Vascular Endothelial Cell Growth Factor) ELISA Kit

Manual
  • Specifications
Product name

Human VEGF(Vascular Endothelial Cell Growth Factor) ELISA Kit

Catalogue No.

EH0327

Alias

VEGF ELISA Kit, VAS ELISA Kit, Vasculotropin ELISA Kit, VEGFA ELISA Kit, VPF ELISA Kit, MVCD1 ELISA Kit, vascular endothelial growth factor A ELISA Kit, Vascular permeability factor ELISA Kit, VEGF-A ELISA Kit, VPFvascular endothelial growth factor ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

VEGF ELISA Kit allows for the in vitro quantitative determination of VEGF concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

31.25-2000pg/ml

Sensitivity

< 18.75pg/ml

Standard curve

Human VEGF(Vascular Endothelial Cell Growth Factor) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of VEGF and the recovery rates were calculated by comparing the measured value to the expected amount of VEGF in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 94-103 98
EDTA plasma(n=5) 87-104 96
heparin plasma(n=5) 87-101 95
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of VEGF and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 90-103% 89-102% 85-102% 88-101%
EDTA plasma(n=5) 89-99% 82-97% 89-97% 88-100%
heparin plasma(n=5) 82-94% 81-98% 81-95% 87-99%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

Service

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Hotline

027-87384275
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