QuickTest ELISA Kits

Traditional sandwich ELISA assay usually takes 3-4 hours. Most detection reagents in the market are concentrated and can't be distinguished from the colour. Dilution is required before use. FineTest developed QuickTest ELISA kits to decrease customers’ operational errors, improve user's experience and get accurate assay result more efficiently. Compared with regular ELISA kits, FineTest QuickTest ELISA kits have more advantages in the same linearity, stability, recovery and storage conditions.

Category

 

Regular ELISA Kit

 

90min Rapid Assay

 

120min Rapid Assay

 

Advantage Analysis

Reaction Duration

 

4h

 

90min

 

120min

 

a great decrease of assay time

Washing Times

 

10

 

5

 

7

 

Less washing, easy operation

Washing Immersion

 

Y

 

N

 

N

 

Faster washing without waiting

Dilution of Detection Reagents

 

Concentrated, Dilution Required

 

Ready-to-Use without dilution

 

Ready-to-Use without dilution

 

Avoid improper reagent preparation

Distinguished Reagent Colour

 

N

 

coloured

 

coloured

 

Avoid missing and mistaking in sample loading, improve loading efficiency

Precision

 

CV<8%

 

CV<6%

 

CV<6%

 

Lower CV, Higher Precision

Numbers of Reagents

 

9

 

6

 

7

 

Less Reagents

1. 90min Rapid Assay(Double antibody-Sandwich ELISA)
1.1. Assay Flowchart

90min Rapid Assay

1.2. Assay Principle

Capture antibody was conjugated to an affinity tag that was recognized by a specific antibody pre-coated on the QuickTest plate. Add the Cap/Det Ab working solution into each well, then add the standards and pilot samples into relevant wells. Mix thoroughly and then incubate. If the sample contains detection target, a capture antibody-detection target-biotin-detection antibody complex was formed. After washing off unbound conjugates, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm with a microplate reader. The concentration of the detection target in the sample was calculated by plotting a standard curve. The concentration of the target substance is proportional to the OD450 value.

1.3. Assay Procedure Summary

Step 1: Take out the required strips, add 50ul Cap/Det Ab into each well, then add 50ul Standard or Sample into relevant well. (When adding standard or sample, the disposable tip lightly touches the liquid level. Change the disposable tips for different samples and standards.) Gently tap the plate for 10s to ensure complete mixing then statically incubate for 60 minutes at 37°C.
Washing: Wash the plate five times without immersion.
Step 2: Add 90ul TMB substrate solution, seal the plate and statically incubate for 10-20 minutes at 37°C(Accurate TMB visualization control is required.).
Step 3: Add 50ul stop solution. Read at 450nm immediately and calculate.

1.4. Kit Components and Storage

No.

 

Item

 

Size(48T)

 

Size(96T)

 

Storage Condition for Opened Kit

E001

 

ELISA Microplate(Dismountable)

 

8×6

 

8×12

 

Put the rest strips into a sealed foil bag with the desiccant. Stored for 1 month at 2-8°C; Stored for 6 month at -20°C

E002

 

Lyophilized Standard

 

1vial

 

2vial

 

Put the rest standards into a desiccant bag. Stored for 1 month at 2-8°C; Stored for 6 month at -20°C

E055

 

Cap/Det Ab

(Ready to use)

 

3ml

 

6ml

 

2-8°C (Avoid Direct Light)

E024

 

TMB Substrate

 

5ml

 

10ml

 

E039

 

Sample Dilution Buffer (blue)

 

20ml

 

20ml

 

2-8°C

E026

 

Stop Solution

 

5ml

 

5ml

 

E038

 

Wash Buffer(25X)

 

15ml

 

30ml

 

E006

 

Plate Sealer

 

3 pieces

 

5 pieces

 

 

E007

 

Product Description

 

1 copy

 

1 copy

 

2. 120min Rapid Assay(Double antibody-Sandwich ELISA)

2.1. Assay Flowchart

120min Rapid Assay

2.2. Assay Principle

Capture antibody was conjugated to an affinity tag that was recognized by a specific antibody pre-coated on the QuickTest plate. Add the Cap/Det Ab working solution into each well, then add the standards and pilot samples into relevant wells. Mix thoroughly and then incubate. If the sample contains detection target, a capture antibody-detection target-biotin-detection antibody complex was formed. After washing off unbound conjugates, HRP-Streptavidin was added to incubate. Repeat the washing process and then TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm with a microplate reader. The concentration of the detection target in the sample was calculated by plotting a standard curve. The concentration of the target substance is proportional to the OD450 value.

2.3. Assay Procedure Summary

Step 1: Take out the required strips, add 50ul Cap/Det Ab into each well, then add 50ul Standard or Sample into relevant well. (When adding standard or sample, the disposable tip lightly touches the liquid level. Change the disposable tips for different samples and standards.) Gently tap the plate for 10s to ensure complete mixing then statically incubate for 60 minutes at 37°C.
Washing: Wash the plate twice without immersion.
Step 2: Add 100ul HRP-Streptavidin (orange) into each well, seal the plate and statically incubate for 30 minutes at 37°C.
Washing: Wash the plate five times without immersion.
Step 3: Add 90ul TMB substrate solution, seal the plate and statically incubate for 10-20 minutes at 37°C(Accurate TMB visualization control is required.).
Step 4: Add 50ul stop solution. Read at 450nm immediately and calculate.

2.4. Kit Components and Storage

No.

 

Item

 

Size(48T)

 

Size(96T)

 

Storage Condition for Opened Kit

E001

 

ELISA Microplate(Dismountable)

 

8×6

 

8×12

 

Put the rest strips into a sealed foil bag with the desiccant. Stored for 1 month at 2-8°C; Stored for 6 month at -20°C

E002

 

Lyophilized Standard

 

1vial

 

2vial

 

Put the rest standards into a desiccant bag. Stored for 1 month at 2-8°C; Stored for 6 month at -20°C

E054

 

Cap/Det Ab

(Ready to use)

 

3ml

 

6ml

 

2-8°C (Avoid Direct Light)

E053

 

HRP-Streptavidin

(Ready to use, orange)

 

5ml

 

10ml

 

E024

 

TMB Substrate

 

5ml

 

10ml

 

E039

 

Sample Dilution Buffer(blue)

 

20ml

 

20ml

 

2-8°C

E026

 

Stop Solution

 

5ml

 

5ml

 

E038

 

Wash Buffer(25X)

 

15ml

 

30ml

 

E006

 

Plate Sealer

 

3 pieces

 

5 pieces

 

 

E007

 

Product Description

 

1 copy

 

1 copy