Flow Cytometry Antibodies

Flow Cytometry(FCM) is the rapid qualitative and quantitative analysis for multi-parameter and sorting technology, using flow cytometer to measure cells or particles irradiated by particular wavelength laser in a fast and straight-line flow state. Detected samples include various cells(e.g. peripheral blood, bone marrow, suspension or adhension cultured cells), supernatant(plasma, serum, cell culture fluid, lysate, biopsy puncture fluid), microorganisms and synthetic microspheres etc.

Applications

Widely used in the fields of immunology, medicine and biology etc, such as cell phenotype identification, cell sorting, cytokine measurement, cell proliferation, cell apoptosis, cell cycle, cell killing etc.

Comparison between Flow Cytometry and ELISA

Assay

 

Antibody

 

Proper Device

 

Marker

 

Reactant

 

Reaction Type

 

Measurement Indicator

 

Result Reading

 

Protein Expression

Flow Cytometry

 

flow cytometry antibody

 

flow cytometer

 

fluorescent substances

 

fluorescent group

 

fluorescence excitation

 

emission

 

fluorescence value

 

Intracellular, cell surface, sorting

ELISA

 

ELISA Antibody Pair

 

microplate reader

 

biotin-streptavidin-HRP

 

TMB

 

enzyme-catalyzed reaction

 

absorbance

 

OD value

 

extracellular

Flow cytometry antibodies are particularly applied in flow cytometry, accurately measuring and quantitatively analyzing for specific proteins on the cell surface or in the cell. Flow cytometry antibodies are widely applied in the fields of biomedical research, clinical diagnosis, drug development and bioengineering etc.

FineTest developed classically cloned and purified antibody with high affinity and specificity based on the monoclonal antibody development platform, covering human, rat and mouse etc. Various targets are available for your choice. Meanwhile, FineTest offers integrative flow cytometry solution including free colour matching, assay guidance and data analysis services etc.

Hot Selling CD Markers

CD3

 

CD3E

 

CD4

 

CD8A

 

CD10

 

CD11A

 

CD11B

 

CD11C

CD14

 

CD15

 

CD16

 

CD18

 

CD19

 

CD20

 

CD22

 

CD25

CD28

 

CD29

 

CD31

 

CD34

 

CD38

 

CD40

 

CD45

 

CD47

CD48

 

CD50

 

CD56

 

CD73

 

CD80

 

CD86

 

CD90

 

CD105

CD107A

 

CD117

 

CD122

 

CD123

 

CD127

 

CD134

 

CD147

 

CD150

CD161

 

CD183

 

CD184

 

CD193

 

CD274

 

CD279

 

 

 

 

Flow Cytometry Colour Matching for Immunocyte Subpopulations

Species

Cell Populations

Flow Cytometry Antibody Combination

Cat.No

Human

T/B/NK cell populations detection

CD45-PerCP

PCP-30039

CD3-FITC

FITC-30004

CD16-PE

PE-30061

CD56-PE

PE-30008

CD19-APC

APC-30066

Human

Thl/Th2 cell populations detection

CD3-PerCP/Cyanine5.5

PCP55-30004

CD4-FITC

FITC-30005

IFN-Y-PE

PE-30053

IL4-APC

APC-30043

Mouse

Thl/Th2 cell populations detection

CD3-PerCP/Cyanine5.5

PCP55-30002

CD4-FITC

FITC-30001

IFN-Y-PE

PE-30074

IL4-APC

APC-30026

Human

Treg cell populations detection

CD4-FITC

FITC-30005

CD25-PE

PE-30035

CD127-APC

APC-30033

Mouse

Treg cell populations detection

CD4-FITC

FITC-30001

CD25-PE

PE-30017

FOXP3-APC

APC-30055

Isotype

Isotype uses immunoglobulin and its subtype to match the primary antibody in the same species, Ig subclass and volume, removing background fluorescence caused by non-specific binding between the antibody and the cell.

A List of Isotype Control Antibodies

Cat.No

Product Name

Host

Marker

Size

FITC-30073

FITC Rat IgG2a,κ Isotype Control(2A3)

Rat

FITC

20T/100T

PE-30073

PE Rat IgG2a,κ Isotype Control(2A3)

Rat

PE

20T/100T

APC-30073

APC Rat IgG2a,κ Isotype Control(2A3)

Rat

APC

20T/100T

FITC-30075

FITC Rat IgG1,κ Isotype Control(HRPN)

Rat

FITC

20T/100T

PE-30075

PE Rat IgG1,κ Isotype Control(HRPN)

Rat

PE

20T/100T

APC-30075

APC Rat IgG1,κ Isotype Control(HRPN)

Rat

APC

20T/100T

FITC-30076

FITC Mouse IgG1,κ Isotype

Control(MOPC-21)

Mouse

FITC

20T/100T

PE-30076

PE Mouse IgG1,κ Isotype

Control(MOPC-21)

Mouse

PE

20T/100T

APC-30076

APC Mouse IgG1,κ Isotype

Control(MOPC-21)

Mouse

APC

20T/100T

PCP-30076

PerCP Mouse IgG1,κ Isotype Control(MOPC-21)

Mouse

PerCP

20T/100T

FITC-30077

FITC Mouse IgG2a,κ Isotype Control(C1.18.4)

Mouse

FITC

20T/100T

PE-30077

PE Mouse IgG2a,κ Isotype Control(C1.18.4)

Mouse

PE

20T/100T

APC-30077

APC Mouse IgG2a,κ Isotype Control(C1.18.4)

Mouse

APC

20T/100T

FITC-30078

FITC Mouse IgG2b,κ Isotype Control(MPC-11)

Mouse

FITC

20T/100T

PE-30078

PE Mouse IgG2b,κ Isotype Control(MPC-11)

Mouse

PE

20T/100T

APC-30078

APC Mouse IgG2b,κ Isotype Control(MPC-11)

Mouse

APC

20T/100T