Products
RBM15 antibody
- Product Name
- RBM15 antibody
- Catalogue No.
- FNab07163
- Size
- 100μg
- Form
- liquid
- Purification
- Protein A+G purification
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- monoclonal
- Isotype
- IgG1
- Clone ID
- 6B1
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- RNA binding motif protein 15
- Alternative Names
- FLJ12479 antibody, FLJ21943 antibody, One twenty two protein 1 antibody, OTT antibody, OTT1 antibody, RBM15 antibody, RNA binding motif protein 15 antibody, SPEN antibody
- UniProt ID
- Q96T37
- Observed MW
- 107 kDa
Application
- Tested Applications
- ELISA, WB, IHC, IF, IP
- Recommended dilution
- WB: 1:500-1:2000; IP: 1:500-1:1000; IHC: 1:200-1:800; IF: 1:10-1:100
Validated Images
HeLa cells were subjected to SDS PAGE followed by western blot with FNab07163(RBM15 antibody) at dilution of 1:1000
IP result of anti-RBM15(IP:FNab07163, 4ug; Detection:FNab07163 1:500) with HEK-293 cells lysate 2600 ug
Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using FNab07163( RBM15 antibody) at dilution of 1:500
Immunofluorescent analysis of Hela cells, using FNab07163( RBM15 antibody) at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red).
- Background
- May function as an mRNA export factor, stimulating export and expression of RTE-containing mRNAs which are present in many retrotransposons that require to be exported prior to splicing. High affinity binding of pre-mRNA to RBM15 may allow targeting of the mRNP to the export helicase DBP5 in a manner that is independent of splicing-mediated NXF1 deposition, resulting in export prior to splicing. May be implicated in HOX gene regulation.
