Human MMP-3(Matrix Metalloproteinase 3) ELISA Kit

Manual
  • Specifications
Product name

Human MMP-3(Matrix Metalloproteinase 3) ELISA Kit

Catalogue No.

EH0235

Alias

MMP-3(Matrix Metalloproteinase 3) ELISA Kit, Stromelysin 1 ELISA Kit, CHDS6 ELISA Kit, proteoglycanase ELISA Kit, SL-1 ELISA Kit, STMY ELISA Kit, STR1 ELISA Kit, stromelysin-1 ELISA Kit, transin-1 ELISA Kit, Transin-1 ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

MMP-3 ELISA Kit allows for the in vitro quantitative determination of MMP-3 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.156-10ng/ml

Sensitivity

< 0.094ng/ml

Standard curve

Human MMP-3(Matrix Metalloproteinase 3) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of MMP-3 and the recovery rates were calculated by comparing the measured value to the expected amount of MMP-3 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 85-100 93
EDTA plasma(n=5) 85-99 93
heparin plasma(n=5) 87-101 95
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MMP-3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 91-104% 87-102% 86-99% 87-97%
EDTA plasma(n=5) 88-98% 90-100% 82-98% 89-99%
heparin plasma(n=5) 80-93% 84-98% 85-98% 80-99%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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