Human MMP-2 (Matrix Metalloproteinase 2) ELISA Kit

Manual
  • Specifications
Product name

Human MMP-2 (Matrix Metalloproteinase 2) ELISA Kit

Catalogue No.

EH0017

Alias

MMP-2( Matrix Metalloproteinase 2) ELISA Kit, Gelatinase A ELISA Kit, 72 kDa gelatinase ELISA Kit, CLG4 ELISA Kit, collagenase type IV-A ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

MMP-2 ELISA Kit allows for the in vitro quantitative determination of MMP-2 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.5-32ng/ml

Sensitivity

< 0.191ng/ml

Standard curve

Human MMP-2 (Matrix Metalloproteinase 2) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of MMP-2 and the recovery rates were calculated by comparing the measured value to the expected amount of MMP-2 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 89-97 94
EDTA plasma(n=5) 86-105 91
heparin plasma(n=5) 86-100 92
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MMP-2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 86-104% 86-105% 91-101% 93-105%
EDTA plasma(n=5) 84-92% 85-98% 82-97% 84-100%
heparin plasma(n=5) 90-99% 90-100% 86-96% 81-99%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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