Human MCP-1(Monocyte Chemotactic Protein 1) ELISA Kit

Manual
  • Specifications
Product name

Human MCP-1(Monocyte Chemotactic Protein 1) ELISA Kit

Catalogue No.

EH0222

Alias

MCP-1 ELISA Kit, CCL2 ELISA Kit, GDCF-2 ELISA Kit, HC11 ELISA Kit, HSMCR30 ELISA Kit, MCAF ELISA Kit, MCP1 ELISA Kit, SCYA2 ELISA Kit, SMC-CF ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

MCP-1 ELISA Kit allows for the in vitro quantitative determination of MCP-1 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

15.625-1000pg/ml

Sensitivity

< 9.375pg/ml

Standard curve

Human MCP-1(Monocyte Chemotactic Protein 1) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of MCP-1 and the recovery rates were calculated by comparing the measured value to the expected amount of MCP-1 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 94-105 100
EDTA plasma(n=5) 86-99 91
heparin plasma(n=5) 85-102 92
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MCP-1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 89-104% 90-97% 87-103% 86-103%
EDTA plasma(n=5) 83-101% 83-97% 87-94% 87-94%
heparin plasma(n=5) 80-98% 83-97% 80-99% 84-100%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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