Human IL-15(Interleukin 15) ELISA Kit

Manual
  • Specifications
Product name

Human IL-15(Interleukin 15) ELISA Kit

Catalogue No.

EH0177

Alias

IL-15 ELISA Kit, IL15 ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

IL-15 ELISA Kit allows for the in vitro quantitative determination of IL-15 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

15.625-1000pg/ml

Sensitivity

< 9.375pg/ml

Standard curve

Human IL-15(Interleukin 15) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of IL-15 and the recovery rates were calculated by comparing the measured value to the expected amount of IL-15 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 86-104 96
EDTA plasma(n=5) 85-103 92
heparin plasma(n=5) 88-102 96
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-15 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 85-104% 88-105% 86-96% 85-101%
EDTA plasma(n=5) 85-100% 82-101% 83-101% 85-100%
heparin plasma(n=5) 92-100% 80-98% 80-98% 81-91%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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