Products
Recombinant Human CD274
- Product Name
- Recombinant Human CD274
- Catalogue No.
- P8899
- Species
- Human
- Host
- HEK293 cells
- Expression Region
- 19-239
- Mol. Weight
- 35-38 KDa
- Tags
- C-terminal His Tag
- Purity
- Greater than 90% as determined by SDS-PAGE
- Application
- Western Blot, ELISA
- Activity
- Measured by its binding ability in a functional ELISA.Immobilized human CD274 at 20 μg/ml (100 μl/well) can bind human PD1 with a linear range of 0.05-0.1 μg/ml.
- Formulation
- Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose and 0.06% proclin, pH7.4
- Reconstitution
- Centrifuge the vial prior to opening, reconstitute in sterile distilled water or buffer of interest to concentration of 0.1-1mg/ml by gently pipetting 2-3 times, don't vortex
- Storage
- The lyophilized protein is stable at -20°C for up to 1 year, the protein solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing 0.1% BSA (make sure BSA will NOT interfere with your experiment ) and store in working aliquots at -20°C to -80°C for 6 months. Avoid repeated freeze/thaw cycle.
- Alternative Names
- B7 H Protein, B7 H1 Protein, B7 homolog 1 Protein, B7H1 Protein, CD274 Protein, CD274 molecule Protein, PD L1 Protein, PDCD1 ligand 1 Protein, PDCD1L1 Protein, PDCD1LG1 Protein, PDL1 Protein
- SDS-PAGE
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