Recombinant Mouse M-CSF

Alternative Names:Colony stimulating factor 1 Protein, Colony stimulating factor 1 (macrophage) Protein, Colony stimulating factor macrophage specific Protein, CSF 1 Protein, CSF-1 Protein, CSF1 Protein, CSF1_HUMAN Protein, Csfm Protein, Lanimostim Protein, M CSF Protein, M-CSF Protein, Macrophage colony stimulating factor Protein, Macrophage Colony Stimulating Factor 1 Protein
Catalogue No.:P4552Uniprot ID:P07141
Host:E.ColiExpression Region:33-187
Mol. Weight:16.9 kDaTags:N-terminal His Tag
  • SPECIFICATIONS
Product Name
Recombinant Mouse M-CSF
Catalogue No.
P4552
Species
Mouse
Host
E.Coli
Expression Region
33-187
Mol. Weight
16.9 kDa
Tags
N-terminal His Tag
Purity
Greater than 95% as determined by SDS-PAGE
Application
Western Blot, ELISA
Activity
Measured in a cell proliferation assay using M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is 0.7-3 ng/mL.
Formulation
Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose, pH7.4
Reconstitution
Centrifuge the vial prior to opening, reconstitute in sterile distilled water or buffer of interest to concentration of 0.1-1mg/ml by gently pipetting 2-3 times, don't vortex
Storage
The lyophilized protein is stable at -20°C for up to 1 year, the protein solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing 0.1% BSA (make sure BSA will NOT interfere with your experiment ) and store in working aliquots at -20°C to -80°C for 6 months. Avoid repeated freeze/thaw cycle.
Alternative Names
Colony stimulating factor 1 Protein, Colony stimulating factor 1 (macrophage) Protein, Colony stimulating factor macrophage specific Protein, CSF 1 Protein, CSF-1 Protein, CSF1 Protein, CSF1_HUMAN Protein, Csfm Protein, Lanimostim Protein, M CSF Protein, M-CSF Protein, Macrophage colony stimulating factor Protein, Macrophage Colony Stimulating Factor 1 Protein
SDS-PAGE