Recombinant SARS-CoV-2 Spike Glycoprotein

Alternative Names:Spike glycoprotein Protein, S glycoprotein Protein
Catalogue No.:P3018Uniprot ID:P0DTC2
Host:HEK293 cellsExpression Region:16-1213
Mol. Weight:180 KDaTags:C-terminal His-Avi Tag
  • SPECIFICATIONS
Product Name
Recombinant SARS-CoV-2 Spike Glycoprotein
Catalogue No.
P3018
Species
Coronavirus
Host
HEK293 cells
Expression Region
16-1213
Mol. Weight
180 KDa
Tags
C-terminal His-Avi Tag
Purity
Greater than 95% as determined by SDS-PAGE
Application
Western Blot, ELISA
Activity
Measured by its binding ability in a functional ELISA with Recombinant Human ACE2 (Catalog # P2955).
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with 5% trehalose, pH7.4
Reconstitution
Centrifuge the vial prior to opening, reconstitute in sterile distilled water or buffer of interest to concentration of 0.1-1mg/ml by gently pipetting 2-3 times, don't vortex
Storage
The lyophilized protein is stable at -20°C for up to 1 year, the protein solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing 0.1% BSA (make sure BSA will NOT interfere with your experiment ) and store in working aliquots at -20°C to -80°C for 6 months. Avoid repeated freeze/thaw cycle.
Alternative Names
Spike glycoprotein Protein, S glycoprotein Protein
SDS-PAGE