Products
WNK4 antibody
- Product Name
- WNK4 antibody
- Catalogue No.
- FNab09516
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- WNK lysine deficient protein kinase 4
- Alternative Names
- PHA2B antibody, PRKWNK4 antibody, WNK4 antibody
- UniProt ID
- Q96J92
- Observed MW
- 45 kDa, 60 kDa
Application
- Tested Applications
- ELISA, WB
- Recommended dilution
- WB: 1:200-1:2000
Validated Images
mouse colon tissue were subjected to SDS PAGE followed by western blot with FNab09516(WNK4 Antibody) at dilution of 1:600
- Background
- Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SCNN1A, SCNN1B, SCNN1D, SGK1, TRPV5 and TRPV6. Regulates the activity of the thiazide-sensitive Na-Cl cotransporter, SLC12A3, by phosphorylation which appears to prevent membrane trafficking of SLC12A3. Also inhibits the renal K(+) channel, KCNJ1, via a kinase-independent mechanism by which it induces clearance of the protein from the cell surface by clathrin-dependent endocytosis. WNK4 appears to act as a molecular switch that can vary the balance between NaCl reabsorption and K(+) secretion to maintain integrated homeostasis. Phosphorylates NEDD4L. Acts as a scaffold to inhibit SLC4A4 as well as CFTR activities and surface expression, recruits STK39 which mediates the inhibition(By similarity).
