Products
NDFIP1 antibody
- Product Name
- NDFIP1 antibody
- Catalogue No.
- FNab05602
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- Nedd4 family interacting protein 1
- Alternative Names
- N4WBP5 antibody
- UniProt ID
- Q9BT67
- Observed MW
- 30 kDa
Application
- Tested Applications
- ELISA, IP, WB, IF
- Recommended dilution
- WB: 1:500-1:2000; IP: 1:200-1:1000; IF: 1:20-1:200
Validated Images
HEK-293 cells were subjected to SDS PAGE followed by western blot with FNab05602( NDFIP1 Antibody) at dilution of 1:300
IP Result of anti-NDFIP1 (IP:FNab05602, 3ug; Detection:FNab05602 1:300) with HEK-293 cells lysate 3200ug.
- Background
- Activates HECT domain-containing E3 ubiquitin-protein ligases, including NEDD4 and ITCH, and consequently modulates the stability of their targets. As a result, controls many cellular processes. Prevents chronic T-helper cells-mediated inflammation by activating ITCH and thus controlling JUNB degradation(By similarity). In cortical neurons, mediates the ubiquitination of SLC11A2/DMT1 by NEDD4L, leading to down-regulation of the divalent metal transporter and protection of the cells from cobalt and iron toxicity(PubMed:19706893). Modulates EGFR signaling through multiple pathways. In particular, may regulate the ratio of AKT1-to-MAPK8 signaling in response to EGF, acting on AKT1 probably through PTEN destabilization and on MAPK8 through ITCH-dependent MAP2K4 inactivation. As a result, may control cell growth rate(PubMed:20534535). Enhances the ubiquitination of BRAT1 mediated by E3 ubiquitin-protein ligases: NEDD4, NEDD4L and ITCH, and is required for the nuclear localization of ubiquitinated BRAT1(PubMed:25631046).
