Abstract: Nitric oxide(NO) is the autocrine and paracrine signalling molecule, which plays an important physiological role in promoting vascular dynamic balance. It can inhibit the contraction and growth of smooth muscle, prevent platelet aggregation, and avoid leukocyte-endothelial cell adhesion. Besides, it's also involved in immune defense system, neurotransmission, angiogenesis etc.
Keywords: Nitric Oxide Cell Signaling, NO Signaling Pathway, Gaseous Signaling Molecules
1. Nitric Oxide Cell Signaling
Nitric oxide(NO) is produced from the L-arginine oxidized by nitric oxide synthase (NOS). The half-life of NO is very short(about 5s). Thus, most researches on NO function are based on the regulation of NOS activity. Different types of NOS exist in various cells. Different mechanisms of signal transduction produce NO. Currently, there are three kinds of NOS, including endothelial tissue(eNOS or NOS3), nervous system(nNOS or NOS1), cytokine-induced type(iNOS or NOS2).
2. Biological Functions of Nitric Oxide
Downstream targets of NO include guanylate cyclase(cGMPase) and NF-κB. cGMPase can improve cGMP level. NF-κB functions as a key transcription factor in iNOS gene expression. The imbalance of NO level and signal in the body usually occurs in some diseases. Diabetic patients have the lowest NO level in the world. Atherosclerosis usually results in the damage of nitric oxide signaling pathway.
3. Cited FineTest Antibody
Publication Details
Article Title: Dibutyl phthalate promotes angiogenesis in EA. hy926 cells through estrogen receptor-dependent activation of ERK1/2, PI3K-Akt, and NO signaling pathways
Journal Title: Chemico-Biological Interactions
DOI: 10.1016/j.cbi.2022.110174
IF: 5.168
PMID: 36089060
| FineTest Product | Sample | Detection Target | Application |
| anti- NOS3 antibody (FNab10162) | EA.hy926 cells | NOS3 | Western Blot |
Validated Image
Figure Source: Chem Biol Interact. 2022 Oct 1;366:110174. doi: 10.1016/j.cbi.2022.110174.
Fig. 5. EA.hy926 cells were exposed to three different concentrations of DBP for (C) 24 h or (D, E) indicated periods of time, after which the cells were collected and the levels of eNOS or phospho-eNOS were assessed using Western blotting, respectively.
4. Recommended Antibodies
| Catalogue No. | Product Name | Specificity | Applications |
| FNab05774 | anti- nNOS antibody | Human, Mouse, Rat | ELISA, WB, IHC |
| FNab04325 | anti- iNOS antibody | Human, Mouse, Rat | ELISA, WB, IHC, IF |
| FNab10777 | anti- iNOS antibody | Human, Rat | ELISA, IHC |
| FNab09945 | anti- NOS3 antibody | Human, Mouse, Rat | ELISA, WB, IHC |
| FNab10162 | anti- NOS3 antibody | Human, Mouse, Rat | ELISA, WB, IHC |
| FNab10931 | anti- Phospho-eNOS-S1177 antibody | Human, Mouse | WB |
5. Supplementary Resources
Fig. S1. NOS1 Signaling Pathway.
Fig. S2. NOS2 Signaling Pathway.
Fig. S3. NOS3 Signaling Pathway.
REFERENCES
[1]Nitric oxide and cyclic GMP signaling pathway as a focus for drug development, PMID: 21728973.
[2]Nitric Oxide Signaling Pathway in Ventral Tegmental Area is Involved in Regulation of 7,8-Dihydroxyflavone on Alcohol Consumption in Rats, PMID: 34618330.
[3]Roles of the nitric oxide signaling pathway in cardiac ischemic preconditioning against myocardial ischemia-reperfusion injury, PMID: 21278703.