[Cited FineTest ELISA Kit] Current Research on Hepatocellular Cancer

FineTest ELISA kit contributes to the research on hepatocellular cancer. The immunoassay is designed to measure 27HC concentration in cells and cell culture medium.

Publication Details
Article Title: TMEM147 aggravates the progression of HCC by modulating cholesterol homeostasis, suppressing ferroptosis, and promoting the M2 polarization of tumor-associated macrophages
Journal Title: Journal of Experimental & Clinical Cancer Research
DOI: 10.1186/s13046-023-02865-0
IF: 11.3
PMID: 37891677

Abstract: We investigated the role of TMEM147 in HCC and the underlying mechanisms. TMEM147 expression was examined in human HCC cells and adjacent non-tumorous tissues using quantitative reverse transcription-polymerase chain reaction, western blotting, and immunohistochemistry. Lipidomic analysis and enzyme-linked immunosorbent assay (ELISA) were employed to determine and analyze cholesterol and 27-hydroxycholesterol (27HC) contents. HCC cell-derived 27HC expression increased the lipid metabolism in macrophages and activated peroxisome proliferator-activated receptor-γ signaling, thereby activating M2 macrophage polarization and promoting HCC cell invasion and migration. Our results indicate that TMEM147 confers ferroptosis resistance and M2 macrophage polarization, which are primarily dependent on the upregulation of cellular cholesterol homeostasis and 27HC secretion, leading to cancer growth and metastasis. These findings suggest that the TMEM147/STAT2/DHCR7/27HC axis in the tumor microenvironment may serve as a promising therapeutic target for HCC.

Keywords: Hepatocellular cancer, Cholesterol metabolites, Ferroptosis, Hepatocellular carcinoma, TMEM147, Tumor-associated macrophages

Immunoassay

Validated Image

Figure Source: J Exp Clin Cancer Res. 2023 Oct 28;42(1):286. doi: 10.1186/s13046-023-02865-0.