[Cited FineTest ELISA Kit] Current Research on Clostridioides Difficile Treatment

FineTest ELISA kit contributes to the research on clostridioides difficile treatment. The immunoassay is designed to measure CXCL1 concentration in the supernatants.

Publication Details
Article Title: A commensal protozoan attenuates Clostridioides difficile pathogenesis in mice via arginine-ornithine metabolism and host intestinal immune response
Journal Title: Nature Communications
DOI: 10.1038/s41467-024-47075-0
IF: 16.6
PMID: 38565558

Abstract: Antibiotic-induced dysbiosis is a major risk factor for Clostridioides difficile infection (CDI), and fecal microbiota transplantation (FMT) is recommended for treating CDI. However, the underlying mechanisms remain unclear. Here, we show that Tritrichomonas musculis (T.mu), an integral member of the mouse gut commensal microbiota, reduces CDI-induced intestinal damage by inhibiting neutrophil recruitment and IL-1β secretion, while promoting Th1 cell differentiation and IFN-γ secretion, which in turn enhances goblet cell production and mucin secretion to protect the intestinal mucosa. T.mu can actively metabolize arginine, not only influencing the host's arginine-ornithine metabolic pathway, but also shaping the metabolic environment for the microbial community in the host's intestinal lumen. This leads to a relatively low ornithine state in the intestinal lumen in C. difficile-infected mice. These changes modulate C. difficile's virulence and the host intestinal immune response, and thus collectively alleviating CDI. These findings strongly suggest interactions between an intestinal commensal eukaryote, a pathogenic bacterium, and the host immune system via inter-related arginine-ornithine metabolism in the regulation of pathogenesis and provide further insights for treating CDI.

Keywords: Clostridioides difficile treatment, Antimicrobial responses, Bacterial host response, Interleukins, Parasite host response, T-helper 1 cells

Immunoassay

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Figure Source: Nat Commun. 2024 Apr 2;15(1):2842. doi: 10.1038/s41467-024-47075-0.