FineTest ELISA kit contributes to the research on traumatic brain injury. The immunoassay is designed to measure TNF-α and IL-6 concentration in the supernatants.
Publication Details
Article Title: Neutrophil Targeting Platform Reduces Neutrophil Extracellular Traps for Improved Traumatic Brain Injury and Stroke Theranostics
Journal Title: Advanced Science
DOI: 10.1002/advs.202308719
IF: 15.1
PMID: 38520727
Abstract: Traumatic brain injuries (TBI) and stroke are major causes of morbidity and mortality in both developing and developed countries. The complex and heterogeneous pathophysiology of TBI and cerebral ischemia-reperfusion injury (CIRI), in addition to the blood-brain barrier (BBB) resistance, is a major barrier to the advancement of diagnostics and therapeutics. Clinical data showed that the severity of TBI and stroke is positively correlated with the number of neutrophils in peripheral blood and brain injury sites. Furthermore, neutrophil extracellular traps (NETs) released by neutrophils correlate with worse TBI and stroke outcomes by impairing revascularization and vascular remodeling. Therefore, targeting neutrophils to deliver NETs inhibitors to brain injury sites and reduce the formation of NETs can be an optimal strategy for TBI and stroke therapy. Herein, the study designs and synthesizes a reactive oxygen species (ROS)-responsive neutrophil-targeting delivery system loaded with peptidyl arginine deiminase 4 (PAD4) inhibitor, GSK484, to prevent the formation of NETs in brain injury sites, which significantly inhibited neuroinflammation and improved neurological deficits, and improved the survival rate of TBI and CIRI. This strategy may provide a groundwork for the development of targeted theranostics of TBI and stroke.
Keywords: neutrophil, neutrophil extracellular traps (NETs), stroke, theranostics, traumatic brain injury
Immunoassay
| FineTest Product | Sample | Species | Detection Target |
| Mouse TNF-α(Tumor Necrosis Factor Alpha) ELISA Kit (EM0183) | supernatants | Mouse | TNF-α |
| Mouse IL-6(Interleukin 6) ELISA Kit(EM0121) | IL-6 |
Validated Image
Figure Source: Adv Sci (Weinh). 2024 Mar 23:e2308719. doi: 10.1002/advs.202308719.