Products
VRK2 antibody
- Product Name
- VRK2 antibody
- Catalogue No.
- FNab09452
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- vaccinia related kinase 2
- Alternative Names
- Vaccinia related kinase 2 antibody, VRK2 antibody
- UniProt ID
- Q86Y07
- Observed MW
- 67 kDa
Application
- Tested Applications
- ELISA, WB, IP
- Recommended dilution
- WB: 1:500-1:5000；IP: 1:200-1:2000
Validated Images
K-562 cells were subjected to SDS PAGE followed by western blot with FNab09452(VRK2 antibody) at dilution of 1:500
IP Result of anti-VRK2 (IP:FNab09452, 4ug; Detection:FNab09452 1:500) with K-562 cells lysate 3600ug.
- Background
- Serine/threonine kinase that regulates several signal transduction pathways. Isoform 1 modulates the stress response to hypoxia and cytokines, such as interleukin-1 beta(IL1B) and this is dependent on its interaction with MAPK8IP1, which assembles mitogen-activated protein kinase(MAPK) complexes. Inhibition of signal transmission mediated by the assembly of MAPK8IP1-MAPK complexes reduces JNK phosphorylation and JUN-dependent transcription. Phosphorylates 'Thr-18' of p53/TP53, histone H3, and may also phosphorylate MAPK8IP1. Phosphorylates BANF1 and disrupts its ability to bind DNA and reduces its binding to LEM domain-containing proteins. Downregulates the transactivation of transcription induced by ERBB2, HRAS, BRAF, and MEK1. Blocks the phosphorylation of ERK in response to ERBB2 and HRAS. Can also phosphorylate the following substrates that are commonly used to establish in vitro kinase activity: casein, MBP and histone H2B, but it is not sure that this is physiologically relevant.Isoform 2 phosphorylates 'Thr-18' of p53/TP53, as well as histone H3. Reduces p53/TP53 ubiquitination by MDM2, promotes p53/TP53 acetylation by EP300 and thereby increases p53/TP53 stability and activity.
