anti- SFN antibody

  • Specifications
  • Manual
  • Contact
Product Information
Product name

anti- SFN antibody

Catalogue No.

FNab09897

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 24 months(Avoid repeated freeze / thaw cycles.)

Background

14-3-3 proteins regulate many cellular processes relevant to cancer biology, notably apoptosis, mitogenic signaling and cell-cycle checkpoints. Seven isoforms, denoted 14-3-3 b, g, e, z, h, q and s, comprise this family of signaling intermediates. 14-3-3 s, also known as SFN, stratifin, HME1 or YWHAS, is a secreted adaptor protein that is involved in regulating both general and specific signaling pathways. Expressed predominately in stratified squamous keratinising epithelium, 14-3-3 s is able to bind and modify the activity of a large number of proteins, such as KRT17 (Keratin 17), through recognition of a phosphothreonine or phosphoserine motif. When bound to Keratin 17, for example, 14-3-3 s acts to stimulate the Akt/mTOR signaling pathway by upregulating protein synthesis and cell growth. 14-3-3 s also functions to positively mediate IGF-I-induced cell cycle progression and can bind to a variety of translation initiation factors, thus controlling mitotic translation. In response to tumor growth, 14-3-3 s positively regulates the tumor suppressor p53 and increases the rate of p53-regulated inhibition of G2/M cell cycle progression. Multiple isoforms of 14-3-3 s exist due to alternative splicing events.

Immunogen Information
Immunogen

14-3-3 protein sigma

Synonyms

14 3 3 protein sigma, HME1, SFN, stratifin, YWHAS

Calculated MW

31 kDa

Application
Specificity

Human, Mouse, Rat

Tested Application

ELISA, WB, IHC, IF

Recommended dilution

WB: 1:500 – 1:2000; IHC: 1:50 – 1:200; IF: 1:50 – 1:100

Uniprot information
Uniprot ID

P31947

Images

anti- SFN antibody 1

Immunohistochemistry of paraffin-embedded rat liver tissue slide using FNab09897( SFN antibody) at dilution of 1:100


anti- SFN antibody 1

MCF-7 cells were subjected to SDS PAGE followed by western blot with FNab09897( SFN antibody) at dilution of 1:1000

Current product's manual: Manual

Protocol may be updated. Please feel free to contact Finetest Team to acquire the latest version.

RetailerEnd User





RetailerEnd User





Service

Hotline

027-87384275
var cnzz_s_tag=document.createElement('script'); cnzz_s_tag.type='text/javascript'; cnzz_s_tag.async=true; cnzz_s_tag.charset='utf-8'; cnzz_s_tag.src='https://w.cnzz.com/c.php?id=1253658600&async=1'; var root_s=document.getElementsByTagName('script')[0]; root_s.parentNode.insertBefore(cnzz_s_tag,root_s);