NDUFB5 antibody

Catalogue No.:FNab05622Reactivity:Human, Mouse, Rat
Host:RabbitTested Application:ELISA, WB, IHC
Clonality:polyclonalIsotype:IgG
  • SPECIFICATIONS
Product Name
NDUFB5 antibody
Catalogue No.
FNab05622
Size
100μg
Form
liquid
Purification
Immunogen affinity purified
Purity
≥95% as determined by SDS-PAGE
Clonality
polyclonal
Isotype
IgG
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months (Avoid repeated freeze / thaw cycles.)
Immunogen
Immunogen
NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 5, 16kDa
UniProt ID
O43674
Observed MW
17 kDa
Application
Tested Applications
ELISA, WB, IHC
Recommended dilution
WB: 1:500 - 1:2000; IHC: 1:50 - 1:100
Validated Images
HepG2 cells were subjected to SDS PAGE followed by western blot with FNab05622(NDUFB5 antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using FNab05622( NDUFB5 Antibody) at dilution of 1:200
Background
The protein encoded by this gene is a subunit of the multisubunit NADH:ubiquinone oxidoreductase (complex I). Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Three transcript variants encoding different isoforms have been found for this gene.