Products
Histone H1.2 antibody
- Product Name
- Histone H1.2 antibody
- Catalogue No.
- FNab03883
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- histone cluster 1, H1c
- Alternative Names
- H1.2 antibody, H1F2 antibody, HIST1H1C antibody, histone cluster 1 antibody, H1c antibody, Histone H1.2 antibody, Histone H1d antibody
- UniProt ID
- P16403
- Observed MW
- 32kd
Application
- Tested Applications
- ELISA, IHC, WB, IF, IP
- Recommended dilution
- WB: 1:500-1:3000; IP: 1:500-1:1000; IHC: 1:20-1:200; IF: 1:10-1:100
Validated Images
L02 cells were subjected to SDS PAGE followed by western blot with FNab03883(HIST1H1C antibody) at dilution of 1:500
IP Result of anti-Histone H1.2 (IP: FNab03883, 3ug; Detection: FNab03883 1:600) with HeLa cells lysate 1200ug.
Immunohistochemical of paraffin-embedded human ovary tumor using FNab03883(HIST1H1C antibody) at dilution of 1:100
Immunofluorescent analysis of Hela cells, using HIST1H1C antibody FNab03883 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
- Background
- Histone H1 regulates chromatin competency through its high affinity binding to linker DNA as a structural component. It acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation. The calcualted molecular weight of Histone H1.2 is 21 kDa, but modified Histone H1.2 is about 32 kDa.
