Products
APEX1 antibody
- Product Name
- APEX1 antibody
- Catalogue No.
- FNab00479
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months (Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- APEX nuclease (multifunctional DNA repair enzyme) 1
- Alternative Names
- APE antibody, APE1 antibody, APEX antibody, APX antibody, HAP1 antibody, REF1 antibody
- UniProt ID
- P27695
- Observed MW
- 39 kDa
Application
- Tested Applications
- ELISA, WB, IP, IF
- Recommended dilution
- WB: 1:500 - 1:2000; IF: 1:50 - 1:100; IP: 1:50 - 1:200
Validated Images
HepG2 cells were subjected to SDS PAGE followed by western blot with FNab00479(APEX1 antibody) at dilution of 1:1000
IP Result of anti-APEX1 (IP:FNab00479, 4ug; Detection:FNab00479 1:1000) with HeLa cells lysate 1600ug.
Immunofluorescence analysis of HeLa cells using FNab00479(APEX1 antibody) at dilution of 1:50
- Background
- Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes the major AP endonuclease in human cells. Splice variants have been found for this gene; all encode the same protein.
