HEK-293 cells were subjected to SDS PAGE followed by western blot with FNab00323(AP2A1 antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded rat kidney using FNab00323(AP2A1 antibody) at dilution of 1:100
Background
This gene encodes the alpha 1 adaptin subunit of the adaptor protein 2 (AP-2) complex found in clathrin coated vesicles. The AP-2 complex is a heterotetramer consisting of two large adaptins (alpha or beta), a medium adaptin (mu), and a small adaptin (sigma). The complex is part of the protein coat on the cytoplasmic face of coated vesicles which links clathrin to receptors in vesicles. Alternative splicing of this gene results in two transcript variants encoding two different isoforms. A third transcript variant has been described, but its full length nature has not been determined.