Recombinant Mouse IL-2

Alternative Names:Aldesleukin Protein, IL 2 Protein, IL2 Protein, IL-2 Protein, interleukin 2 Protein, lymphokine Protein, T cell growth factor Protein, TCGF Protein
Catalogue No.:P8599Uniprot ID:P04351
Host:E.ColiExpression Region:21-169
Mol. Weight:37.9 kDaTags:N-terminal His-IF2DI Tag
  • SPECIFICATIONS
Product Name
Recombinant Mouse IL-2
Catalogue No.
P8599
Species
Mouse
Host
E.Coli
Expression Region
21-169
Mol. Weight
37.9 kDa
Tags
N-terminal His-IF2DI Tag
Purity
Greater than 95% as determined by SDS-PAGE
Application
Western Blot, ELISA
Activity
Measured in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells. The ED50 for this effect is 0.5-1 ng/mL.
Formulation
Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose and 0.06% proclin, pH7.4
Reconstitution
Centrifuge the vial prior to opening, reconstitute in sterile distilled water or buffer of interest to concentration of 0.1-1mg/ml by gently pipetting 2-3 times, don't vortex
Storage
The lyophilized protein is stable at -20°C for up to 1 year, the protein solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing 0.1% BSA (make sure BSA will NOT interfere with your experiment ) and store in working aliquots at -20°C to -80°C for 6 months. Avoid repeated freeze/thaw cycle.
Alternative Names
Aldesleukin Protein, IL 2 Protein, IL2 Protein, IL-2 Protein, interleukin 2 Protein, lymphokine Protein, T cell growth factor Protein, TCGF Protein
SDS-PAGE