FineTest Elisa kit contributes to the research on fat metabolism and obesity. The immunoassay is designed to measure cytokines concentration (Adiponectin, TNF-α, IL-1β, IL-6) in serum.
Publication Details
Article Title: Gab2 Deficiency Suppresses High-fat Diet-induced Obesity by Reducing Adipose Tissue Inflammation and Increasing Brown Adipose Function in Mice
Journal Title: Cell Death & Disease
DOI: 10.1038/s41419-021-03519-9
IF: 10.717
PMID: 33637697
Abstract: Obesity is caused by a long-term imbalance between energy intake and consumption and is regulated by multiple signals. This study investigated the effect of signaling scaffolding protein Gab2 on obesity and its relevant regulation mechanism. Gab2 knockout (KO) and wild-type (WT) mice were fed with a standard diet (SD) or high-fat diet (HFD) for 12 weeks. The results showed that the a high-fat diet-induced Gab2 expression in adipose tissues, but deletion of Gab2 attenuated weight gain and improved glucose tolerance in mice fed with a high-fat diet. White adipose tissue and systemic inflammations were reduced in HFD-fed Gab2 deficiency mice. Gab2 deficiency increased the expression of Ucp1 and other thermogenic genes in brown adipose tissue. Furthermore, the regulation of Gab2 on the mature differentiation and function of adipocytes was investigated in vitro using primary or immortalized brown preadipocytes. The expression of brown fat-selective genes was found to be elevated in differentiated adipocytes without Gab2. The mechanism of Gab2 regulating Ucp1 expression in brown adipocytes involved with its downstream PI3K (p85)-Akt-FoxO1 signaling pathway. Our research suggests that deletion of Gab2 suppresses diet-induced obesity by multiple pathways and Gab2 may be a novel therapeutic target for the treatment of obesity and associated complications.
Immunoassay
| FineTest Product | Sample | Detection Target | Species |
| Mouse TNF-α(Tumor Necrosis Factor Alpha) ELISA Kit (EM0183) | serum | TNF-α | |
| Mouse IL-6(Interleukin 6) ELISA Kit (EM0121) | IL-6 | ||
| Mouse IL-1β(Interleukin 1 Beta) ELISA Kit (EM0109) | IL-1β | Mouse | |
| Mouse ADP/Acrp30(Adiponectin) ELISA Kit (EM0001) | Adiponectin | ||
| Recombinant Mouse Leptin (P0191) | Leptin |
Validated Image
Figure Source: Cell Death Dis, 2021 Feb 26;12(2):212. doi: 10.1038/s41419-021-03519-9.
Fig. 4: Gab2 deficiency reduces macrophage infiltration and inflammation in adipose tissue in HFD-fed mice.
A Representative immunohistochemistry (IHC) staining images of F4/80 protein in eWAT from Gab2 KO and WT mice fed with HFD diet for 12 weeks. Crown-like structures are indicated by the red arrows. Scale bars are 100 μm (top) and 50 μm (bottom). B Quantification of crown-like structure (CLS) in eWAT from Gab2 KO and WT mice fed with HFD diet for 12 weeks. C The mRNA level of F4/80 and Mcp-1 in eWAT from Gab2 KO and WT mice fed with SD or HFD diet for 12 weeks were detected by RT-qPCR. The results were normalized with S18 mRNA levels. D Representative picro-sirius staining images of collagen fibrils in eWAT from Gab2 KO and WT mice fed with HFD diet for 12 weeks. Collagen fibrils are indicated by the red arrows. Scale bars are 100 μm (top) and 50 μm (bottom). E The mRNA level of adiponectin (Adipoq), leptin, and inflammation cytokines (Tnf-α, Il-1β, Il-6) in eWAT from Gab2 KO and WT mice fed with HFD diet for 12 weeks detected by RT-qPCR. The results were normalized with S18 mRNA levels. F–H The concentration of adiponectin (F), leptin (G), and inflammation cytokines (TNF-α, IL-1β, IL-6, H) in blood plasma samples analyzed by ELISA assay. The data are presented as mean ± SD from at least 6 mice in each group. Statistical difference is indicated: *P < 0.05; **P < 0.01; ***P < 0.001.