[Cited FineTest ELISA Kit] Current Research on Nanofiber Wound Dressing

FineTest ELISA kit contributes to the research on nanofiber wound dressing. The immunoassay is designed to measure lysozyme concentration in supernatant.

Publication Details
Article Title: Long-term antibacterial activity by synergistic release of biosafe lysozyme and chitosan from LBL-structured nanofibers
Journal Title: Carbohydrate Polymers
DOI: 10.1016/j.carbpol.2023.120791
IF: 10.723
PMID: 37059531

Abstract:      Biosafe antibacterial agents are urgently demanded in treating infection especially chronic infection. However, efficient and controlled release of those agents remains great challenging. Two nature-derived agents, lysozyme (LY) and chitosan (CS), are selected to establish a facile method for long-term bacterial inhibition. We incorporated LY into the nanofibrous mats, then deposited CS and polydopamine (PDA) on the surface by layer-by-layer (LBL) self-assembly. In this vein, LY is gradually released with the degradation of nanofibers, and CS is rapidly disassociated from the nanofibrous mats to synergistically result in a potent inhibition against Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) over a period of 14 days. Besides long-term antibacterial capacity, LBL-structured mats could readily achieve a strong tensile stress of 6.7 MPa with an increase percentage of up to 103%. The enhanced proliferation of L929 cells arrives at 94% with help of CS and PDA on the surface of nanofibers. In this vein, our nanofiber has a variety of advantages including biocompatibility, strong long-term antibacterial effect, and skin adaptability, revealing the significant potential to be used as highly safe biomaterial for wound dressings.

Keywords:    Chitosan, Layer-by-layer, Long-term antibacterial activity, Lysozyme, Nanofiber

Immunoassay

Illustration

Figure Source:  Carbohydr Polym. 2023 Jul 15;312:120791. doi: 10.1016/j.carbpol.2023.120791.