FineTest ELISA kit contributes to the research on cerebral amyloid angiopathy treatment. The immunoassay is designed to measure NEFL and C5b-9 concentration in plasma.
Publication Details
Article Title: Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse model
Journal Title: Nature Communications
DOI: 10.1038/s41467-023-39693-x
IF: 17.69
PMID: 37402721
Abstract: Accumulation of amyloid beta protein (Aβ) in brain vessels damages blood brain barrier (BBB) integrity in cerebral amyloid angiopathy (CAA). Macrophage lineage cells scavenge Aβ and produce disease-modifying mediators. Herein, we report that Aβ40-induced macrophage-derived migrasomes are sticky to blood vessels in skin biopsy samples from CAA patients and brain tissue from CAA mouse models (Tg-SwDI/B and 5xFAD mice). We show that CD5L is packed in migrasomes and docked to blood vessels, and that enrichment of CD5L impairs the resistance to complement activation. Increased migrasome-producing capacity of macrophages and membrane attack complex (MAC) in blood are associated with disease severity in both patients and Tg-SwDI/B mice. Of note, complement inhibitory treatment protects against migrasomes-mediated blood-brain barrier injury in Tg-SwDI/B mice. We thus propose that macrophage-derived migrasomes and the consequent complement activation are potential biomarkers and therapeutic targets in CAA.
Keywords: Complement cascade, Monocytes and macrophages, Neurovascular disorder
Immunoassay
| FineTest Product | Sample | Species | Detection Target |
| Mouse NEFL(Neurofilament light polypeptide) ELISA Kit(EM1688) | Plasma | Mouse | NEFL |
| Mouse TCC C5b-9(Terminal Complement Complex C5b-9) ELISA Kit(EM1392) | Mouse | C5b-9 | |
| Human TCC C5b-9 (Terminal Complement Complex C5b-9)ELISA Kit(EH4763) | Human | C5b-9 |
Validated Image
Figure Source: Nat Commun. 2023 Jul 4;14(1):3945. doi: 10.1038/s41467-023-39693-x