FineTest ELISA kit contributes to the research on cancer drug resistance. The immunoassay is designed to measure CCL5 and AREG concentration in cell supernatant.
Publication Details
Article Title: Macrophage-organoid co-culture model for identifying treatment strategies against macrophage-related gemcitabine resistance
Journal Title: Journal of Experimental & Clinical Cancer Research
DOI: 10.1186/s13046-023-02756-4
IF: 11.3
PMID: 37553567
Abstract: Gemcitabine resistance (GR) is a significant clinical challenge in pancreatic adenocarcinoma (PAAD) treatment. To investigate the effects of macrophage stimulation on GR, co-cultures were set up using PDOs from three PAAD patients with macrophages. The macrophage-CCL5-Sp1-AREG feedback loop between macrophages and PCCs is responsible for GR. The results from the PDO model were corroborated with cell lines, mouse models, and clinical data. Our study highlights that the PDO model is a superior choice for preclinical research and precision medicine. The macrophage-CCL5-Sp1-AREG feedback loop confers stemness to PCCs to facilitate gemcitabine resistance by activating the CCR5/AKT/SP1/CD44 pathway. The combination of gemcitabine and mithramycin shows potential as a therapeutic strategy for treating PAAD in cell lines, PDOs, and mouse models.
Keywords: Cancer stem cells, Gemcitabine, Cancer Drug Resistance, Macrophages, Organoids
Immunoassay
| FineTest Product | Sample | Species | Detection Target |
| Human Rantes(Regulated On Activation, Normal T-Cell Expressed and Secreted) ELISA Kit(EH0265) | cell supernatant | human | CCL5 |
| Human AREG(Amphiregulin) ELISA Kit(EH6284) | AREG |
Validated Image
Figure Source: J Exp Clin Cancer Res. 2023 Aug 9;42(1):199. doi: 10.1186/s13046-023-02756-4.
Fig. 2. The quantitative analysis of AREG (left) and CCL5 (right) by ELISA in different cells of the two models after 3 days of culture. (n = 3).