FineTest Elisa kit contributes to the research on metabolic disorder. The immunoassay is designed to measure 3-methyl histidine concentration in plasma.
Publication Details
Article Title: Is Muscle and Protein Loss Relevant in Long-term Fasting in Healthy Men? A Prospective Trial on Physiological Adaptations
Journal Title: Journal of Cachexia, Sarcopenia and Muscle
DOI: 10.1002/jcsm.12766
IF: 12.91
PMID: 34668663
Abstract: Fasting is attracting an increasing interest as a potential strategy for managing diseases, including metabolic disorders and complementary cancer therapy. Despite concerns of clinicians regarding protein catabolism and muscle loss, evidence-based clinical data in response to long-term fasting in healthy humans are scarce. The objective of this study was to measure clinical constants, metabolic, and muscular response in healthy men during and after a 10 day fast combined with a physical activity programme.
Keywords: Apelin, Body Composition, Energy Metabolism, Lipids, Long-term Fasting, Muscle Function, Metabolic Disorder
Immunoassay
| FineTest Product | Sample | Detection Target | Species |
| Human 3MH(3-methylhistidine) ELISA Kit (EH4250) | Plasma | 3MH | Human |
Validated Image
Figure Source: J Cachexia Sarcopenia Muscle, 2021 Dec;12(6):1690-1703. doi: 10.1002/jcsm.12766.
Figure 2. Changes in body weight (BW), fat mass (FM), and lean soft tissues (LST) at F09 vs. baseline. LST change is estimated as the loss of metabolically active tissues (MAT), glycogen (Gly), and extracellular water (ECW) (A). Measurement of protein oxidation (B), urea excretion (C), creatinine excretion (D), plasma ASAT (E), plasma ALAT (F), plasma 3 methyl histidine (G), myostatin (H), 13CO2 of expired air (I) and plasma apelin (J) before and during the 10 day fasting (n = 16) and after 3 months of Fup (n = 15). Data are lsmeans ± SE. ALAT, alanine amino transferase; ASAT, aspartate amino transferase; F, fasting days; Fup, follow-up; RF, food reintroduction days. *P < 0.05, **P < 0.01, ***P < 0.001 as indicated by the lines for the whole effect of fasting, the whole effect of RF or the effect of Fup vs. the end of fasting. ###P < 0.01 vs. baseline during Fup.