Products

POLR1A antibody

Synonyms:A190 antibody, POLR1A antibody, RNA polymerase I subunit A1 antibody, RPA1 antibody, RPA194 antibody, RPO1 4 antibody
Catalogue No.:FNab06615Reativity:Human
Host:RabbitTested Application:ELISA, WB
Clonality:polyclonalIsotype:IgG
  • SPECIFICATIONS
Product Name
POLR1A antibody
Catalogue No.
FNab06615
Size
100μg
Form
liquid
Purification
Immunogen affinity purified
Purity
≥95% as determined by SDS-PAGE
Clonality
polyclonal
Isotype
IgG
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
Immunogen
polymerase(RNA) I polypeptide A, 194kDa
Alternative Names
A190 antibody, POLR1A antibody, RNA polymerase I subunit A1 antibody, RPA1 antibody, RPA194 antibody, RPO1 4 antibody
UniProt ID
O95602
Observed MW
190-195 kDa
Application
Tested Applications
ELISA, WB
Recommended dilution
WB: 1:500-1:2000
Validated Images
HeLa cells were subjected to SDS PAGE followed by western blot with FNab06615(POLR1A antibody) at dilution of 1:1000
Background
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase I which synthesizes ribosomal RNA precursors. Forms the polymerase active center together with the second largest subunit. A single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol I. A bridging helix emanates from RPA1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol I by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition(By similarity).