Human WISP1/CCN4(WNT1-inducible-signaling pathway protein 1) ELISA Kit

说明书
  • 规格
Product name

Human WISP1/CCN4(WNT1-inducible-signaling pathway protein 1) ELISA Kit

Catalogue No.

EH0330

Alias

WISP1 ELISA Kit, WISP-1 ELISA Kit, CCN4 ELISA Kit, WISP-1i ELISA Kit, CCN4WISP1tc ELISA Kit, CCN family member 4 ELISA Kit, Wnt-1-induced secreted protein ELISA Kit, WISP1c ELISA Kit, WNT1 induced secreted protein 1 ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

WISP1/CCN4 ELISA Kit allows for the in vitro quantitative determination of WISP1/CCN4 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

31.2-2000pg/ml

Sensitivity

< 18.75pg/ml

Standard curve

Human WISP1/CCN4(WNT1-inducible-signaling pathway protein 1) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of WISP1/CCN4 and the recovery rates were calculated by comparing the measured value to the expected amount of WISP1/CCN4 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 87-103 93
EDTA plasma(n=5) 85-96 92
heparin plasma(n=5) 85-101 93
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of WISP1/CCN4 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 90-105% 87-105% 85-100% 86-93%
EDTA plasma(n=5) 83-91% 84-99% 83-100% 85-99%
heparin plasma(n=5) 80-99% 80-98% 87-100% 89-94%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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