Human VTCN1(V-set domain-containing T-cell activation inhibitor 1) ELISA Kit

说明书
  • 规格
Product name

Human VTCN1(V-set domain-containing T-cell activation inhibitor 1) ELISA Kit

Catalogue No.

EH1214

Alias

VTCN1 ELISA Kit, V-set domain-containing T-cell activation inhibitor 1 ELISA Kit, T-cell costimulatory molecule B7x ELISA Kit, Protein B7S1 ELISA Kit, B75 ELISA Kit, Immune costimulatory protein B7-H4 ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

VTCN1 ELISA Kit allows for the in vitro quantitative determination of VTCN1 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

7.8-500ng/ml

Sensitivity

< 4.688ng/ml

Standard curve

Human VTCN1(V-set domain-containing T-cell activation inhibitor 1) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of VTCN1 and the recovery rates were calculated by comparing the measured value to the expected amount of VTCN1 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 86-100 94
EDTA plasma(n=5) 85-100 91
heparin plasma(n=5) 88-99 94
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of VTCN1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 87-105% 85-103% 87-105% 85-97%
EDTA plasma(n=5) 91-96% 84-98% 86-98% 82-101%
heparin plasma(n=5) 94-100% 81-100% 86-99% 87-98%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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