Human SPARCL1(SPARC-like protein 1) ELISA Kit

说明书
  • 规格
Product name

Human SPARCL1(SPARC-like protein 1) ELISA Kit

Catalogue No.

EH0275

Alias

SPARCL1(SPARC Like Protein 1) ELISA Kit, Hevin ELISA Kit, MAST9 ELISA Kit, SC1 ELISA Kit, Hevin ELISA Kit, High endothelial venule protein ELISA Kit, MAST 9 ELISA Kit, mast9 ELISA Kit, PIG33 ELISA Kit, proliferation-inducing protein 33 ELISA Kit, SC1 ELISA Kit, SPARC-like 1(mast9, hevin) ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

SPARCL1 ELISA Kit allows for the in vitro quantitative determination of SPARCL1 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.313-20ng/ml

Sensitivity

< 0.188ng/ml

Standard curve

Human SPARCL1(SPARC-like protein 1) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of SPARCL1 and the recovery rates were calculated by comparing the measured value to the expected amount of SPARCL1 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 86-105 96
EDTA plasma(n=5) 85-104 97
heparin plasma(n=5) 87-103 94
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SPARCL1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 85-102% 86-103% 91-105% 87-102%
EDTA plasma(n=5) 82-100% 89-101% 87-94% 82-101%
heparin plasma(n=5) 85-94% 84-99% 81-93% 86-99%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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