Human SHH(Sonic hedgehog protein) ELISA Kit

说明书
  • 规格
Product name

Human SHH(Sonic hedgehog protein) ELISA Kit

Catalogue No.

EH0605

Alias

SHH ELISA Kit, Shh ELISA Kit, HHG1 ELISA Kit, HHG-1 ELISA Kit, HLP3 ELISA Kit, HPE3 ELISA Kit, SMMCIsonic hedgehog homolog(Drosophila) ELISA Kit, sonic hedgehog ELISA Kit, sonic hedgehog homolog ELISA Kit, sonic hedgehog protein ELISA Kit, TPT ELISA Kit, TPTPS ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

SHH ELISA Kit allows for the in vitro quantitative determination of SHH concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

78.125-5000pg/ml

Sensitivity

< 46.875pg/ml

Standard curve

Human SHH(Sonic hedgehog protein) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of SHH and the recovery rates were calculated by comparing the measured value to the expected amount of SHH in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 89-102 93
EDTA plasma(n=5) 93-103 97
heparin plasma(n=5) 89-105 96
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SHH and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 85-105% 93-99% 86-105% 92-103%
EDTA plasma(n=5) 82-98% 87-97% 84-96% 84-99%
heparin plasma(n=5) 87-93% 81-99% 83-96% 81-100%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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