Human RAC1(Ras Related C3 Botulinum Toxin Substrate 1) ELISA Kit

说明书
  • 规格
Product name

Human RAC1(Ras Related C3 Botulinum Toxin Substrate 1) ELISA Kit

Catalogue No.

EH0100

Alias

MGC111543, MIG5, TC-25, p21-Rac1, migration-inducing gene 5|ras-related C3 botulinum toxin substrate 1|rho family, small GTP binding protein Rac1 ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

RAC1 ELISA Kit allows for the in vitro quantitative determination of RAC1 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.156-10ng/ml

Sensitivity

< 0.094ng/ml

Standard curve

Human RAC1(Ras Related C3 Botulinum Toxin Substrate 1) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of RAC1 and the recovery rates were calculated by comparing the measured value to the expected amount of RAC1 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 89-101 94
EDTA plasma(n=5) 85-95 91
heparin plasma(n=5) 85-95 92
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of RAC1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 86-104% 92-105% 85-105% 86-102%
EDTA plasma(n=5) 83-101% 83-100% 86-100% 83-98%
heparin plasma(n=5) 88-99% 89-96% 82-99% 90-100%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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