Human PRAME(Melanoma antigen preferentially expressed in tumors) ELISA Kit

说明书
  • 规格
Product name

Human PRAME(Melanoma antigen preferentially expressed in tumors) ELISA Kit

Catalogue No.

EH1290

Alias

PRAME(Melanoma antigen preferentially expressed in tumors) ELISA Kit, MAPE ELISA Kit, OIP4 ELISA Kit, OIP-4 ELISA Kit, CT130 ELISA Kit, Cancer,testis antigen 130 ELISA Kit, Opa-interacting protein 4 ELISA Kit, Preferentially expressed antigen of melanoma ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

PRAME ELISA Kit allows for the in vitro quantitative determination of PRAME concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.156-10ng/ml

Sensitivity

< 0.094ng/ml

Standard curve

Human PRAME(Melanoma antigen preferentially expressed in tumors) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of PRAME and the recovery rates were calculated by comparing the measured value to the expected amount of PRAME in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 93-103 98
EDTA plasma(n=5) 89-102 94
heparin plasma(n=5) 94-102 98
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PRAME and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 85-105% 85-103% 87-99% 85-101%
EDTA plasma(n=5) 84-91% 86-101% 82-101% 83-96%
heparin plasma(n=5) 89-99% 82-99% 84-96% 84-100%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

服务

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热线

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