Human PON1(Serum paraoxonase/arylesterase 1) ELISA Kit

说明书
  • 规格
Product name

Human PON1(Serum paraoxonase/arylesterase 1) ELISA Kit

Catalogue No.

EH0257

Alias

PON1(Paraoxonase 1) ELISA Kit, ESA ELISA Kit, K-45 ELISA Kit, A-esterase 1 ELISA Kit, Aromatic esterase 1 ELISA Kit, arylesterase B-type ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

PON1 ELISA Kit allows for the in vitro quantitative determination of PON1 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.156-10ng/ml

Sensitivity

< 0.094ng/ml

Standard curve

Human PON1(Serum paraoxonase/arylesterase 1) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of PON1 and the recovery rates were calculated by comparing the measured value to the expected amount of PON1 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 86-100 96
EDTA plasma(n=5) 89-101 93
heparin plasma(n=5) 88-103 97
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PON1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 87-104% 85-95% 87-100% 87-104%
EDTA plasma(n=5) 84-98% 83-94% 82-97% 84-96%
heparin plasma(n=5) 81-100% 80-98% 88-98% 80-99%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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