Human PMCHL1(Putative pro-MCH-like protein 1) ELISA Kit

说明书
  • 规格
Product name

Human PMCHL1(Putative pro-MCH-like protein 1) ELISA Kit

Catalogue No.

EH1305

Alias

PMCHL1(Putative pro-MCH-like protein 1) ELISA Kit, Pro-MCH variant ELISA Kit, Pro-melanin-concentrating hormone-like protein 1 ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

PMCHL1 ELISA Kit allows for the in vitro quantitative determination of PMCHL1 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

78-5000pg/ml

Sensitivity

< 46.9pg/ml

Standard curve

Human PMCHL1(Putative pro-MCH-like protein 1) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of PMCHL1 and the recovery rates were calculated by comparing the measured value to the expected amount of PMCHL1 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 92-99 95
EDTA plasma(n=5) 88-95 91
heparin plasma(n=5) 90-104 96
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PMCHL1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 86-104% 85-105% 91-104% 90-104%
EDTA plasma(n=5) 84-95% 82-101% 82-100% 82-98%
heparin plasma(n=5) 83-98% 83-96% 82-97% 81-95%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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