Human PDGF-BB(Platelet Derived Growth Factor-BB) ELISA Kit

说明书
  • 规格
Product name

Human PDGF-BB(Platelet Derived Growth Factor-BB) ELISA Kit

Catalogue No.

EH0254

Alias

PDGF-BB(Platelet Derived Growth Factor-BB) ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

PDGF-BB ELISA Kit allows for the in vitro quantitative determination of PDGF-BB concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

31.25-2000pg/ml

Sensitivity

< 18.75pg/ml

Standard curve

Human PDGF-BB(Platelet Derived Growth Factor-BB) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of PDGF-BB and the recovery rates were calculated by comparing the measured value to the expected amount of PDGF-BB in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 92-101 96
EDTA plasma(n=5) 87-104 94
heparin plasma(n=5) 88-104 97
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PDGF-BB and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 91-102% 88-105% 93-102% 90-100%
EDTA plasma(n=5) 82-92% 84-91% 82-101% 83-101%
heparin plasma(n=5) 82-90% 81-97% 88-97% 81-88%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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