Human PCK1(Phosphoenolpyruvate carboxykinase, cytosolic [GTP]) ELISA Kit

说明书
  • 规格
Product name

Human PCK1(Phosphoenolpyruvate carboxykinase, cytosolic [GTP]) ELISA Kit

Catalogue No.

EH1085

Alias

PCK1(Phosphoenolpyruvate Carboxykinase 1) ELISA Kit, PEPCK1 ELISA Kit, PEPCK-C ELISA Kit, PEPCKC ELISA Kit, PEPCK-CPEP carboxykinase ELISA Kit, phosphoenolpyruvate carboxykinase 1(soluble) ELISA Kit, phosphoenolpyruvate carboxykinase, cytosolic ELISA Kit, phosphoenolpyruvate carboxykinase, cytosolic [GTP] ELISA Kit, Phosphoenolpyruvate carboxylase ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

PCK1 ELISA Kit allows for the in vitro quantitative determination of PCK1 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.156-10ng/ml

Sensitivity

< 0.094ng/ml

Standard curve

Human PCK1(Phosphoenolpyruvate carboxykinase, cytosolic [GTP]) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of PCK1 and the recovery rates were calculated by comparing the measured value to the expected amount of PCK1 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 90-104 96
EDTA plasma(n=5) 91-99 97
heparin plasma(n=5) 85-99 90
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PCK1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 86-98% 86-100% 86-101% 87-93%
EDTA plasma(n=5) 87-96% 88-101% 85-100% 85-100%
heparin plasma(n=5) 84-97% 85-100% 84-97% 88-97%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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