Human NDN(Necdin) ELISA Kit

说明书
  • 规格
Product name

Human NDN(Necdin) ELISA Kit

Catalogue No.

EH0866

Alias

NDN ELISA Kit, Necdin ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

NDN ELISA Kit allows for the in vitro quantitative determination of NDN concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.312-20ng/ml

Sensitivity

< 0.188ng/ml

Standard curve

Human NDN(Necdin) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of NDN and the recovery rates were calculated by comparing the measured value to the expected amount of NDN in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 89-105 98
EDTA plasma(n=5) 87-101 95
heparin plasma(n=5) 86-105 97
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NDN and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 86-104% 85-98% 87-101% 89-105%
EDTA plasma(n=5) 87-101% 82-98% 83-101% 85-95%
heparin plasma(n=5) 80-93% 81-99% 80-97% 80-99%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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