Human MMP-9(Matrix Metalloproteinase 9) ELISA Kit

说明书
  • 规格
Product name

Human MMP-9(Matrix Metalloproteinase 9) ELISA Kit

Catalogue No.

EH0238

Alias

MMP-9(Matrix Metalloproteinase 9) ELISA Kit, CLG4B ELISA Kit, Gelatinase B ELISA Kit, GELB ELISA Kit, CLG4B ELISA Kit, macrophage gelatinase ELISA Kit, MANDP2 ELISA Kit, MMP-9 ELISA Kit, type V collagenase ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

MMP-9 ELISA Kit allows for the in vitro quantitative determination of MMP-9 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.313-20ng/ml

Sensitivity

< 0.188ng/ml

Standard curve

Human MMP-9(Matrix Metalloproteinase 9) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of MMP-9 and the recovery rates were calculated by comparing the measured value to the expected amount of MMP-9 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 90-104 99
EDTA plasma(n=5) 87-105 96
heparin plasma(n=5) 87-100 95
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MMP-9 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 91-105% 88-102% 86-101% 92-105%
EDTA plasma(n=5) 87-98% 83-99% 82-100% 88-101%
heparin plasma(n=5) 82-99% 82-99% 83-93% 82-99%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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