Human MIF(Macrophage migration inhibitory factor) ELISA Kit

说明书
  • 规格
Product name

Human MIF(Macrophage migration inhibitory factor) ELISA Kit

Catalogue No.

EH0016

Alias

MIF(Macrophage migration inhibitory factor) ELISA Kit, GIFmacrophage migration inhibitory factor ELISA Kit, GLIF ELISA Kit, Glycosylation-inhibiting factor ELISA Kit, L-dopachrome isomerase ELISA Kit, L-dopachrome tautomerase ELISA Kit, macrophage migration inhibitory factor(glycosylation-inhibiting factor) ELISA Kit, MMIF ELISA Kit, Phenylpyruvate tautomerase ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

MIF ELISA Kit allows for the in vitro quantitative determination of MIF concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.781-50ng/ml

Sensitivity

< 0.469ng/ml

Standard curve

Human MIF(Macrophage migration inhibitory factor) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of MIF and the recovery rates were calculated by comparing the measured value to the expected amount of MIF in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 85-98 92
EDTA plasma(n=5) 86-96 91
heparin plasma(n=5) 93-103 97
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MIF and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 96-105% 91-102% 89-101% 87-104%
EDTA plasma(n=5) 82-89% 85-100% 82-92% 84-100%
heparin plasma(n=5) 81-97% 81-90% 87-98% 81-98%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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