Human LIF(Leukemia inhibitory factor) ELISA Kit

说明书
  • 规格
Product name

Human LIF(Leukemia inhibitory factor) ELISA Kit

Catalogue No.

EH0507

Alias

LIF(Leukemia Inhibitory Factor) ELISA Kit, CDF ELISA Kit, D factor ELISA Kit, DIA ELISA Kit, differentiation inhibitory activity ELISA Kit, differentiation stimulating factor ELISA Kit, Differentiation-stimulating factor ELISA Kit, Emfilermin ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

LIF ELISA Kit allows for the in vitro quantitative determination of LIF concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

31.25-2000pg/ml

Sensitivity

< 18.75pg/ml

Standard curve

Human LIF(Leukemia inhibitory factor) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of LIF and the recovery rates were calculated by comparing the measured value to the expected amount of LIF in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 91-102 95
EDTA plasma(n=5) 88-103 96
heparin plasma(n=5) 85-100 94
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of LIF and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 85-105% 85-100% 86-103% 86-105%
EDTA plasma(n=5) 82-100% 85-101% 84-98% 85-101%
heparin plasma(n=5) 82-100% 82-95% 81-98% 80-94%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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