Human IGFBP-3(Insulin-like growth factor-binding protein 3) ELISA Kit

说明书
  • 规格
Product name

Human IGFBP-3(Insulin-like growth factor-binding protein 3) ELISA Kit

Catalogue No.

EH0169

Alias

IGFBP-3 ELISA Kit, IGFBP3 ELISA Kit, BP-53 ELISA Kit, IBP3 ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

IGFBP-3 ELISA Kit allows for the in vitro quantitative determination of IGFBP-3 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.781-50ng/ml

Sensitivity

< 0.469ng/ml

Standard curve

Human IGFBP-3(Insulin-like growth factor-binding protein 3) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of IGFBP-3 and the recovery rates were calculated by comparing the measured value to the expected amount of IGFBP-3 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 87-104 98
EDTA plasma(n=5) 94-105 100
heparin plasma(n=5) 88-101 96
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IGFBP-3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 90-105% 85-105% 88-104% 91-103%
EDTA plasma(n=5) 82-88% 82-100% 85-98% 86-98%
heparin plasma(n=5) 85-98% 84-97% 80-93% 82-98%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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