Human HK1(Hexokinase-1) ELISA Kit

说明书
  • 规格
Product name

Human HK1(Hexokinase-1) ELISA Kit

Catalogue No.

EH1044

Alias

HK1(Hexokinase-1) ELISA Kit, Brain form hexokinase ELISA Kit, Hexokinase type I ELISA Kit, HK I ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

HK1 ELISA Kit allows for the in vitro quantitative determination of HK1 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

78-5000pg/ml

Sensitivity

< 46.9pg/ml

Standard curve

Human HK1(Hexokinase-1) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of HK1 and the recovery rates were calculated by comparing the measured value to the expected amount of HK1 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 86-105 96
EDTA plasma(n=5) 86-99 94
heparin plasma(n=5) 87-102 95
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HK1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 86-93% 85-104% 92-103% 88-102%
EDTA plasma(n=5) 86-95% 82-96% 82-88% 83-96%
heparin plasma(n=5) 84-98% 83-100% 85-96% 83-99%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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