Human HDAC8(Histone deacetylase 8) ELISA Kit

说明书
  • 规格
Product name

Human HDAC8(Histone deacetylase 8) ELISA Kit

Catalogue No.

EH1258

Alias

HDAC8(Histone deacetylase 8) ELISA Kit, CDA07 ELISA Kit, HDACL1 ELISA Kit, RPD3 ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

HDAC8 ELISA Kit allows for the in vitro quantitative determination of HDAC8 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.625-40ng/ml

Sensitivity

< 0.375ng/ml

Standard curve

Human HDAC8(Histone deacetylase 8) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of HDAC8 and the recovery rates were calculated by comparing the measured value to the expected amount of HDAC8 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 88-105 95
EDTA plasma(n=5) 86-102 93
heparin plasma(n=5) 89-103 94
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HDAC8 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 85-101% 95-104% 85-94% 91-100%
EDTA plasma(n=5) 87-101% 86-96% 87-101% 88-100%
heparin plasma(n=5) 84-99% 85-97% 88-100% 80-96%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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