Human GFAP(Glial fibrillary acidic protein) ELISA Kit

说明书
  • 规格
Product name

Human GFAP(Glial fibrillary acidic protein) ELISA Kit

Catalogue No.

EH0410

Alias

GFAP ELISA Kit, FLJ45472 ELISA Kit, glial fibrillary acidic protein ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

GFAP ELISA Kit allows for the in vitro quantitative determination of GFAP concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.313-20ng/ml

Sensitivity

< 0.188ng/ml

Standard curve

Human GFAP(Glial fibrillary acidic protein) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of GFAP and the recovery rates were calculated by comparing the measured value to the expected amount of GFAP in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 88-104 93
EDTA plasma(n=5) 86-105 99
heparin plasma(n=5) 88-105 95
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GFAP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 88-104% 98-104% 85-102% 85-105%
EDTA plasma(n=5) 86-98% 82-86% 83-100% 85-97%
heparin plasma(n=5) 86-99% 89-100% 80-97% 83-97%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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