Human FN1(Fibronectin) ELISA Kit

说明书
  • 规格
Product name

Human FN1(Fibronectin) ELISA Kit

Catalogue No.

EH0134

Alias

FN(Fibronectin) ELISA Kit, FN1 ELISA Kit, CIG ELISA Kit, ED-B ELISA Kit, FINC ELISA Kit, FNZ ELISA Kit, GFND ELISA Kit, GFND2 ELISA Kit, LETS ELISA Kit, MSF ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

FN1 ELISA Kit allows for the in vitro quantitative determination of FN1 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

1.563-100ng/ml

Sensitivity

< 0.938ng/ml

Standard curve

Human FN1(Fibronectin) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of FN1 and the recovery rates were calculated by comparing the measured value to the expected amount of FN1 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 86-103 92
EDTA plasma(n=5) 90-101 94
heparin plasma(n=5) 85-97 93
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of FN1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 85-94% 93-105% 93-105% 93-103%
EDTA plasma(n=5) 90-100% 82-98% 86-91% 85-94%
heparin plasma(n=5) 84-99% 80-98% 84-99% 80-100%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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