Human FGF1(Heparin-binding growth factor 1 ) ELISA Kit

说明书
  • 规格
Product name

Human FGF1(Heparin-binding growth factor 1 ) ELISA Kit

Catalogue No.

EH0501

Alias

AFGF ELISA Kit, FGF1(Acidic Fibroblast Growth Factor 1) ELISA Kit, alpha-ECGF ELISA Kit, beta-ECGF ELISA Kit, FGF-1 ELISA Kit, HBGF-1 ELISA Kit, AFGF ELISA Kit, aFGF ELISA Kit, ECGFB ELISA Kit, FGFABeta-endothelial cell growth factor ELISA Kit, FGF-alpha ELISA Kit, fibroblast growth factor 1(acidic) ELISA Kit, HBGF1 ELISA Kit, HBGF-1 ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

FGF1 ELISA Kit allows for the in vitro quantitative determination of FGF1 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

31.25-2000pg/ml

Sensitivity

< 18.75pg/ml

Standard curve

Human FGF1(Heparin-binding growth factor 1 ) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of FGF1 and the recovery rates were calculated by comparing the measured value to the expected amount of FGF1 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 89-105 96
EDTA plasma(n=5) 88-102 95
heparin plasma(n=5) 89-100 94
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of FGF1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 92-98% 86-105% 85-104% 90-103%
EDTA plasma(n=5) 84-101% 83-101% 82-99% 82-95%
heparin plasma(n=5) 82-98% 83-89% 82-100% 86-100%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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